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Isolation of Mindanao Bacillus thuringiensis (Berliner) strains and preliminary bioassay against lepidopteran species / Marivique N. Diansuy

By:

Diansuy, Marivique

Material type: Text

TextLanguage: English Publication details: 2002Description: 28 leavesSubject(s):

Undergraduate Thesis BIO200

Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2002 Summary: Soil and leaf samples collected from Davao City, Bukidnon and Compostela Valley were suspended in Luria-broth-sodium acetate solution, heat-treated, diluted and spread-plated onto L-agar medium. After 3-5 days of incubation at room temperature, colonies typical of Bacillus thuringiensis (Bt) were observed. These colonies were transferred and streak-plated onto T3 medium and grown at the same condition. A total of 28 Bt isolates were obtained. Among these, 21 isolates came from soil samples. The protein concentration of spore-crystalline suspensions of 20 randomly selected isolates were determined and preliminary bioassay was carried out against the diamond back moth (Phutella xylostella) larvae using suspensions containing 2g/ml protein. Seven isolates produced 100% mortality after 7 days incubation, five of which were isolated from cornfields relatively free from corn borers. It is recommended that more thorough bioassays be conducted and that the Bt isolates be characterized further in order to determine which specific Cry toxin proteins are produced by the isolates.

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Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	University Library	Non-Circulation	LG993.5 2002 B4 D53 (Browse shelf(Opens below))	Available		3UPML00022061
Thesis	University Library	Reference/Room-Use Only	LG993.5 2002 B4 D53 (Browse shelf(Opens below))	Available		3UPML00010441

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Thesis (BS Biology) -- University of the Philippines Mindanao, 2002

Soil and leaf samples collected from Davao City, Bukidnon and Compostela Valley were suspended in Luria-broth-sodium acetate solution, heat-treated, diluted and spread-plated onto L-agar medium. After 3-5 days of incubation at room temperature, colonies typical of Bacillus thuringiensis (Bt) were observed. These colonies were transferred and streak-plated onto T3 medium and grown at the same condition. A total of 28 Bt isolates were obtained. Among these, 21 isolates came from soil samples. The protein concentration of spore-crystalline suspensions of 20 randomly selected isolates were determined and preliminary bioassay was carried out against the diamond back moth (Phutella xylostella) larvae using suspensions containing 2g/ml protein. Seven isolates produced 100% mortality after 7 days incubation, five of which were isolated from cornfields relatively free from corn borers. It is recommended that more thorough bioassays be conducted and that the Bt isolates be characterized further in order to determine which specific Cry toxin proteins are produced by the isolates.

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