Isolation of Mindanao Bacillus thuringiensis (Berliner) strains and preliminary bioassay against lepidopteran species / (Record no. 218)
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000 -LEADER | |
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fixed length control field | 01718nam a2200205 4500 |
001 - CONTROL NUMBER | |
control field | UPMIN-00000009144 |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20220921140839.0 |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | DLC |
Transcribing agency | DLC |
Modifying agency | upmin |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | eng |
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2002 |
Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 D53 |
100 1# - MAIN ENTRY--PERSONAL NAME | |
Personal name | Diansuy, Marivique . |
245 00 - TITLE STATEMENT | |
Title | Isolation of Mindanao Bacillus thuringiensis (Berliner) strains and preliminary bioassay against lepidopteran species / |
Statement of responsibility, etc. | Marivique N. Diansuy |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Date of publication, distribution, etc. | 2002 |
300 ## - PHYSICAL DESCRIPTION | |
Extent | 28 leaves |
502 ## - DISSERTATION NOTE | |
Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2002 |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Soil and leaf samples collected from Davao City, Bukidnon and Compostela Valley were suspended in Luria-broth-sodium acetate solution, heat-treated, diluted and spread-plated onto L-agar medium. After 3-5 days of incubation at room temperature, colonies typical of Bacillus thuringiensis (Bt) were observed. These colonies were transferred and streak-plated onto T3 medium and grown at the same condition. A total of 28 Bt isolates were obtained. Among these, 21 isolates came from soil samples. The protein concentration of spore-crystalline suspensions of 20 randomly selected isolates were determined and preliminary bioassay was carried out against the diamond back moth (Phutella xylostella) larvae using suspensions containing 2g/ml protein. Seven isolates produced 100% mortality after 7 days incubation, five of which were isolated from cornfields relatively free from corn borers. It is recommended that more thorough bioassays be conducted and that the Bt isolates be characterized further in order to determine which specific Cry toxin proteins are produced by the isolates. |
658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
Main curriculum objective | Undergraduate Thesis |
Curriculum code | BIO200, |
Source of term or code | BSB |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
a | Fi |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
a | UP |
Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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University Library | University Library | 2022-09-21 | UAR-T-gd416 | LG993.5 2002 B4 D53 | 3UPML00022061 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
University Library | University Library | 2022-09-21 | CSM-T-1079 | LG993.5 2002 B4 D53 | 3UPML00010441 | 2022-09-21 | 2022-09-21 | Thesis |