000			01878nam a2200205 4500
001			UPMIN-00000009391
005			20220921142336.0
041			_aeng
040			_aDLC _cDLC _dupmin
090			_aLG993.5 2003 _bB4 P56
100	1		_aPioquinto, Rowena Ruby G.
245	0	0	_aCellulase activity detected in Aspergillus Sp. and metarrhizium sp. isolated from insects / _cRowena Ruby G. Pioquinto
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2003
300			_a44 leaves
260			_c2003
520			_aTwelve entomogenous fungi collected from Compostela Valley, Davao oriental and Tugbok District, Davao City and isolated from jewel beetle, long-horned beetle, rhinoceros beetle, grasshopper, housefly, katydid, and wasp by a previous researcher were screed for cellulose activity, specifically for the presence of three types of cellulose enzymes namely, endogucanase, exoglucanase and -glucosidase. Trichoderma harzianum served as the control. Ten were verified as aspergillus sp. while two were Metarrhizium sp. all thirteen entomogenous fungi, including the control, showed red zone or clearing for the agar diffusion assay but only seven including the control, were identified as potentially high cellulolytic enzyme producer. These were Sla (Aspergillus isolated from rhinoceros beetle), Slc (Aspergillus isolated from grasshopper 2). S2a (metarrhizium isolated from rhinoceros beetle), and S2b (Metarrhizium isolated from grasshopper). The six fungal isolates exhibited cellulose activity comparable to the control. In filter paper assay, fungal isolates S2a and S1b of genus Metarrhizium and the control had the largest amount of cellobiohydrolase enzymes. On the other hand, fungal isolate S1a had the largest amount of avicelase enzymes among all isolates.
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
999			_c362 _d362