000			02346nam a2200241 4500
001			UPMIN-00000009302
003			UPMIN
005			20240530162516.0
008			240530b |||||||| |||| 00| 0 eng d
040			_aDLC _cUPMin _dupmin
041			_aeng
090			_aLG993.5 2004 _bB4 A43
100	1		_aAldaba, Hena S. _eauthor _926032
245	0	0	_aEffects of different levels of plant growth regulators on callus induction of sago palm (Metroxylon sagu Rottb.) shoot tip / _cHena S. Aldaba
260			_c2004
300			_a41 leaves
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2004
520	3		_aMetroxylon sagu Rottb. produces huge amount of starch. The use of tissue culture in sago is beneficial since it has the potential to produce thousands of plantlets in a short period of time. Prior to inoculation to their specific media, they should each pass through sterilization treatment, which minimized the degree of contamination in cultures. Sago shoot tips were first exposed to different commercial chlorine concentrations (5%, 10%, 15%, 20%) at different times of exposure (5, 10, 15, 0 minutes) to find out the optimum combination for sterilization. A high percent recovery was found at 15% concentration at 20 minutes. However, there was no statistical significance between chlorine concentration and time of exposure. Then, the explants were cultured to different media with different concentrations of plant growth regulators, specifically, auxin and cytokinin, for callus initiation. The use of these plant growth regulators enhanced callus formation and shoot elongation. The auxin used was 2, 4-dichlorophenoxyacetic aced (2,4-D) at 0-50, and 100 ppm. The cytokinin used was N6-(2-isopentyl) adenine (2-iP) with concentrations of 0, 0.3, and 0.5 ppm. The optimum treatment combination was treatment 5 and 50 ppm 2,4-D and 0.3 ppm 2-iP. The presence of 2,4-D at this level resulted to a high mean percent callus formation. However, at higher than 50ppm 2,4-D, less callus formation was observed because of its inhibitory effect. Meanwhile, at 0.3 ppm 2-iP showed a high weight increase due to shoot formation. Callus formation and shoot elongation contributed significantly to weight inc
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c329 _d329