000			02220nam a2200205 4500
001			UPMIN-00000009208
005			20220921140844.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2002 _bB4 J35
100	1		_aJamiri, Al-radjid J.
245	0	0	_aThe use of simple sequence repeats (SSR) marker technique to assess genetic diversity in abaca (Musa textilis Nee) / _cAl-radjid J. Jamiri
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2002
300			_a62 leaves
260			_c2002
520			_aThe genetic diversity analysis of abaca was done to compare the different accessions at the National Abaca Research Center (MARC), Leyte. The analysis was done using SSR technique. Ninety-four accessions were analyzed. Twenty-eight Musa SSR primers were used and surveyed for diversity analysis. Out of the seven primers that were optimized during the preliminary screening, only three primers (Ma 3/103, Ma 2/4 and Ma 1/016, were used for genetic diversity analysis. They proved to be useful and applicable for genetic diversity analysis of abaca. Out of 90 accessions, only 64 were used in the analysis. There were 33 alleles that were detected for the three primers, 21 of them were common alleles and 12 were rare alleles. For Ma 3/103, the frequencies of observed bands ranged from 1-11%, while Ma 2/4 3-36%, and Ma 1/016 2-25%. This showed that SSRs or microsatellites occur in abaca genome and that some Musa SSR primers can be used for abaca. There were 24 single-accession clusters and seven clusters with 2-9 identical accessions within cluster that were identified. The SSR data showed that a high degree of variability exists among the accessions collected from Leyte, Samar and Laguna that were used in this study. They also showed that the clusters did not correspond to the geographical origins of the accessions. They further showed that a high degree of duplication occurs in the collection. While these three primers proved to be useful in genetic diversity analysis, more primers should be optimized to ascertain that redundant accessions exist in the collection of NARC.
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
999			_c268 _d268