000			01430nam a2200193 4500
001			UPMIN-00000009202
005			20220921140843.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2000 _bB4 M35
100	1		_aMalicsi, Myra Sharon .
245	0	0	_aOptimization of a Microwell cytotoxicity assay using Artemia salina (brine shrimps) / _cMyra Sharon Malicsi
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2000
300			_a25 leaves
260			_c2000
520			_aThe microwell cytotoxicity assay using Artemia salina (brine shrimps) was done as follows: (1) Artemia salina cysts, 0.25 grams, was hatched in 100 ml artificially prepared seawater supplemented with 0.006 grams of yeast extract, (2) continuous moderate aeration was provided in the culture media, (3) serial dilutions of chloramphenicol, cyclohexanone and vincristine were made in the well of the 96-microwell plate, (4) a suspension containing 10-15 nauplii was added tp each well. (5) after 24 hours of hydration, the number of dead nauplii per well was counted, and (6) media lethal concentration was determined using the Probit analysis. It was found that efficiency and rate of hatching increased with salt concentration in the culture media. The 24 hr. LC50 of chloramphenicol, cyclohexanone and vincristine were 39450.12 mg/ml, 5007.907 mg/ml and 1023.712 mg/ml, respectively
658			_aFi
905			_aUP
999			_c262 _d262