000			01470nam a2200205 4500
001			UPMIN-00000009194
005			20220921140843.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2000 _bB4 F337
100	1		_aFadriquela, Liza F.
245	0	0	_aIn vitro clonal propagation of Phalaenopsis using floral nodes / _cLiza F. Fadriquela
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2000
300			_a21 leaves
260			_c2000
520			_aThis study has two parts, the establishment of sterilization procedure and the node culture stage. In the sterilization stage, nodes were subjected to different concentrations of Clorox (5.25% Na hypochlorite) at different time intervals. Data obtained showed that 15% Clorox for 30 min and 20% Clorox for 15 to 30 min were effective in reducing the contamination rate. In the node culture stage, floral nodes of young floral stalks were inoculated in Vacin and Went medium to develop initial protocols for in vitro culture techniques of Phalaenopsis and to determine which node locations successfully produce shoots. Node locations 1 to 5 were able to produce shoots. The highest average length of shoots was obtained from node location 3. Statistical analysis showed the nodes 1, 2 and 5 were significantly different from nodes 3 and 4 at 5% level of significance in terms of shoot production
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
999			_c255 _d255