000			01374nam a2200205 4500
001			UPMIN-00000009193
005			20220921140843.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2000 _bB4 F47
100	1		_aFernandez, Cathy F.
245	0	0	_aMicropropagation of Vanda sanderiana ("Waling-waling") using root tips : _bI. Initial studies on the standardization of sterilization technique and selection of suitable culture media / _cCathy F. Fernandez
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2000
300			_a25 leaves
260			_c2000
520			_aRoot tips from mature and clonally-propagated V. senderiana were subjected to different Clorox treatments ranging from 5% for 15 and 30 minutes and 10 to 20% for 5-15 minutes. Data obtained showed that mature root tip explants may be sterilized using 10 to 20% clorox for 5 minutes. Tests confirmed that clonally-propagated explants neednot be sterilized. Three types of media were evaluated: namely the modified White?s, modified Vacin-Went and modified Murashige and Skoog. Explants cultured in Vacin-Went medium showed positive growth signs through elongation but results were not sufficient to conclude that it is most suitable for root tip culture of V. sanderiana
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
999			_c254 _d254