000			01565nam a2200205 4500
001			UPMIN-00000009166
005			20220921140841.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2001 _bB4 R39
100	1		_aRazo, Lloyd Fyl O.
245	0	0	_aMicrowell cytotoxicity assay of Gliricidia Sepium (JACQ.) Steud. extract using brine shrimp Artemia Salina L. / _cLloyd O. Razo
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2001
300			_a30 leaves
260			_c2001
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
520			_aThe microwell cytotoxicity assay of madre de cacao gliricidia sepium (Jacq.) Steud extract using brine shrimp, artemia salina L. was done to determine its cytotoxic properties. According the Herath et al. (1998). G. sepium has natural cancer ? protective compound in the form of isoflavonoid, especially called afrormosin. As isoflavonoids contain OH attached to benzene ring their structures, they are considered as derivatives of phenols. Various plant parts (seeds, leaves and branches) were extracted through decoction and were indirectly tested for the presence of phenols. The seed extract tested positive to the phenol test and was used in the cytotoxicity test. Concentrations of 1 ppt and 100 ppt were prepared and placed in the wells of the 96-well microwell plate containing 100 L A. salina nauplii suspension. Mortality rate was determined after 24 hours. Twenty-four (24) hour LC50 of madre de cacao seed extract was found to be 8.6 ppt
999			_c239 _d239