000			01900nam a2200241 4500
001			UPMIN-00000009165
003			UPMIN
005			20240530164819.0
008			240530b |||||||| |||| 00| 0 eng d
040			_aDLC _cUPMin _dupmin
041			_aeng
090			_aLG993.5 2002 _bB4 A56
100	1		_aAno-os, Katherine A. _eauthor _926044
245	0	0	_aComparative microwell cytotoxicity assay of some Philippine plant extracts using brine shrimp Artemia Salina L. / _cKatherine A. Ano-os
260			_c2002
300			_a51 leaves
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2002
520	3		_aCytotoxicity assay was employed using a 96-microwell plate in order to determine the cytotoxic activity of the leaf extracts of Jatropha curcas, Diospyros blancoi, Areca catechu, Ehretia microphylla and Quisqualis indica. In the cytotoxicity test, wells containg nauolii were mixed with different concentrations (10ppm, 100ppm and 1000 ppm) of leaf extracts of the five test plants. Mortality rates were observed after 24 hours. Results showed that the average median lethal concentration of the leaf extracts of J. curcas and A. catechu was 60.34 ppm and 464.05 ppm respectively. LC50 was undetermined for the other three plants since they were not able to cause 50% mortality rate of the brine shrimp nauplii. On the other hand, phenol test was also done in the different leaf extracts on order to detect the probable presence of phenol. Among the five plants, only leaf extract of J. curcas tested positive for the presence of phenol. Thus, it can be concluded that J. curcas leaf extract may contain phenolic compounds that can cause mortality of the brine shrimps nauplii. Substances other than phenols may also cause mortality although at a lower rate.
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
942			_2lcc _cTHESIS
999			_c238 _d238