000			01718nam a2200205 4500
001			UPMIN-00000009144
005			20220921140839.0
040			_aDLC _cDLC _dupmin
041			_aeng
090			_aLG993.5 2002 _bB4 D53
100	1		_aDiansuy, Marivique .
245	0	0	_aIsolation of Mindanao Bacillus thuringiensis (Berliner) strains and preliminary bioassay against lepidopteran species / _cMarivique N. Diansuy
502			_aThesis (BS Biology) -- University of the Philippines Mindanao, 2002
300			_a28 leaves
260			_c2002
658			_aUndergraduate Thesis _cBIO200, _2BSB
905			_aFi
905			_aUP
520			_aSoil and leaf samples collected from Davao City, Bukidnon and Compostela Valley were suspended in Luria-broth-sodium acetate solution, heat-treated, diluted and spread-plated onto L-agar medium. After 3-5 days of incubation at room temperature, colonies typical of Bacillus thuringiensis (Bt) were observed. These colonies were transferred and streak-plated onto T3 medium and grown at the same condition. A total of 28 Bt isolates were obtained. Among these, 21 isolates came from soil samples. The protein concentration of spore-crystalline suspensions of 20 randomly selected isolates were determined and preliminary bioassay was carried out against the diamond back moth (Phutella xylostella) larvae using suspensions containing 2g/ml protein. Seven isolates produced 100% mortality after 7 days incubation, five of which were isolated from cornfields relatively free from corn borers. It is recommended that more thorough bioassays be conducted and that the Bt isolates be characterized further in order to determine which specific Cry toxin proteins are produced by the isolates.
999			_c218 _d218