Normal view MARC view ISBD view

Screening, isolation and identification of mercury resistant bacteria at Apokon, Tagum City / Ivy Lorraine Pagaduan

By:

Pagaduan, Ivy Lorraine

Material type: Text

TextLanguage: English Publication details: 2000Description: 20 leavesSubject(s):

Undergraduate Thesis BIO200

Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2000 Summary: This study aimed to find mercury-resistant bacteria which can be used as pollution indicators by cytochemical staining. Three mercury resistant bacterial isolates (A, B and C) were obtained from a ballmill dumpsite at Apokon, Tagum City. Isolates A and C were identified as Pseudomonas fluorescens while isolate B was identified as Pseudomonas putida. The isolates were grown in nutrient agar media containing varied concentrations of mercury (10, 15 and 20 ppm HgNO3). After three days, it was found that the number of bacteria that survived decreased with increasing HgNO3 concentration. However, as the end of the incubation period was reached, the number of bacteria increased. It was also observed that when bacteria have accumulated mercury, the surface of their colonies turned blue. The results indicate that the isolates possess some mechanisms of detoxifying the mercury-containing environment

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings ( 2 )
Title notes ( 2 )
Comments ( 0 )

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	University Library	Non-Circulation	LG993.5 2000 B4 P34 (Browse shelf(Opens below))	Available		3UPML00020739
Thesis	University Library	Reference/Room-Use Only	LG993.5 2000 B4 P34 (Browse shelf(Opens below))	Available		3UPML00011000

Browsing University Library shelves Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	LG993.5 2000 B4 M357 Growth, reproduction and age determination of seagrass Cymodocea rotundata (Ehrenberg and Hemprich ex ascherson) population in Penaplata, Island Garden City of Samal /	LG993.5 2000 B4 M47 Approximation of microbial load of beef using resazurin dye reduction technique /	LG993.5 2000 B4 M67 The use of float technique in bell pepper (Capsicum annuum L. var annuum) seedling production /	LG993.5 2000 B4 P34 Screening, isolation and identification of mercury resistant bacteria at Apokon, Tagum City /	LG993.5 2000 B4 P45 Morphometrics of the Malayan black bug Scotinophora Coarctata (F.) populations from Zamboanga and Cotabato /	LG993.5 2000 B4 P453 Pollen collection pattern of the honeybee (Apis mellifera L.) /	LG993.5 2000 B4 R36 Meat and bone dust as culture medium for Pediococcus acidilactici /	Next

Thesis (BS Biology) -- University of the Philippines Mindanao, 2000

This study aimed to find mercury-resistant bacteria which can be used as pollution indicators by cytochemical staining. Three mercury resistant bacterial isolates (A, B and C) were obtained from a ballmill dumpsite at Apokon, Tagum City. Isolates A and C were identified as Pseudomonas fluorescens while isolate B was identified as Pseudomonas putida. The isolates were grown in nutrient agar media containing varied concentrations of mercury (10, 15 and 20 ppm HgNO3). After three days, it was found that the number of bacteria that survived decreased with increasing HgNO3 concentration. However, as the end of the incubation period was reached, the number of bacteria increased. It was also observed that when bacteria have accumulated mercury, the surface of their colonies turned blue. The results indicate that the isolates possess some mechanisms of detoxifying the mercury-containing environment

There are no comments on this title.

to post a comment.