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Effect of plant growth regulators on the in vitro culture of Vanda Sanderiana Reichb.f. leaf explants / Jamaica P. Lota

By:

Lota, Jamaica P

Material type: Text

TextLanguage: English Publication details: 2002Description: 31 leavesSubject(s):

Undergraduate Thesis BIO200

Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2002 Summary: Leaf bases, leaf tips and whole leaves were used as explants and inoculated onto Knodson C media with varying concentrations of BAP and NAA: (a) 1mg/l BAP, (b) 1 mg/1NAA, (c) 0.1 mg/l NAA + 0.5 mg/l BAP and; (d) 0.1 mg/l BAP + 0.5 mg/1 NAA. The leaf base was found to be a potential leaf explant for in vitro micropropagation of V. sanderiana. Roots and shoots were formed when inoculated in the control set-up but was assumed to have been a response to wounding. Concentration of cytokinin higher than 1 mg/l is required for the formation of well-developed shoots. Higher concentration of auxin relative to cytokinin led to simultaneous callus and root formation

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Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	University Library	Non-Circulation	LG993.5 2002 B4 L67 (Browse shelf(Opens below))	Available		3UPML00020781
Thesis	University Library	Reference/Room-Use Only	LG993.5 2002 B4 L67 (Browse shelf(Opens below))	Available		3UPML00010989

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Thesis (BS Biology) -- University of the Philippines Mindanao, 2002

Leaf bases, leaf tips and whole leaves were used as explants and inoculated onto Knodson C media with varying concentrations of BAP and NAA: (a) 1mg/l BAP, (b) 1 mg/1NAA, (c) 0.1 mg/l NAA + 0.5 mg/l BAP and; (d) 0.1 mg/l BAP + 0.5 mg/1 NAA. The leaf base was found to be a potential leaf explant for in vitro micropropagation of V. sanderiana. Roots and shoots were formed when inoculated in the control set-up but was assumed to have been a response to wounding. Concentration of cytokinin higher than 1 mg/l is required for the formation of well-developed shoots. Higher concentration of auxin relative to cytokinin led to simultaneous callus and root formation

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