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In vitro clonal propagation of Dieffenbachia sp. : I. initial studies on sterilization of explants and selection of culture media / Sharon Mae D. Atienza

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Atienza, Sharon Mae D [author]

Material type: Text

TextLanguage: English Publication details: 2000Description: 23 leavesSubject(s):

Undergraduate Thesis BIO200

Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2000 Abstract: Stem disc explants of Dieffenbachia sp. were subjected to different Clorox concentrations ranging from 5 to 15% for 10 to 20 minutes. Results showed that the most effective treatment was 10% Clorox for 15 minutes. A modified Murashige and Skoog medium containing (a) 1 mg/l benzylaminopurine (BA), (b) 0.1 mg/l naphthaleneacetic acid (NAA) + 2.5 mg/l BA, or (c) 0.2 mg/l BA was used. No shoots were observed indicating that a much longer time is needed for shoot induction. Other factors such as plant growth regulator (PGR) combinations and age of explant can also affect shoot emergence. Whether the proper concentration of PGRs in the medium is achieved should also be taken into account.

List(s) this item appears in: BS Biology

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Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	University Library Theses	Room-Use Only	LG993.5 2000 B4 A28 (Browse shelf(Opens below))	Available		3UPML00010973
Thesis	University Library Archives and Records	Non-Circulating	LG993.5 2000 B4 A28 (Browse shelf(Opens below))	Preservation Copy		3UPML00020758

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Thesis (BS Biology) -- University of the Philippines Mindanao, 2000

Stem disc explants of Dieffenbachia sp. were subjected to different Clorox concentrations ranging from 5 to 15% for 10 to 20 minutes. Results showed that the most effective treatment was 10% Clorox for 15 minutes. A modified Murashige and Skoog medium containing (a) 1 mg/l benzylaminopurine (BA), (b) 0.1 mg/l naphthaleneacetic acid (NAA) + 2.5 mg/l BA, or (c) 0.2 mg/l BA was used. No shoots were observed indicating that a much longer time is needed for shoot induction. Other factors such as plant growth regulator (PGR) combinations and age of explant can also affect shoot emergence. Whether the proper concentration of PGRs in the medium is achieved should also be taken into account.

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