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Screening of bioactive agents in Moringa Oleifera Lam. seeds using brine shrimp lethality and selected bacteria / Patty S. Buencosa

By:

Buenacosa, Patty S

Material type: Text

TextLanguage: English Publication details: 2001Description: 27 leavesSubject(s):

Undergraduate Thesis BIO200

Dissertation note: Thesis (BS Biology) -- University of the Philippines Mindanao, 2001 Summary: A crude extract from the seeds of Moringa oleifera Lam locally known as malunggay was obtained using chloroform as solvent and twice purified using benzene. The final crude extract was obtained from the benzene layer after evaporation at room temperature. Cytotoxicity test using brine shrimp lethality was done with the extract diluted to the following concentrations: 1 ppt, 10 ppt and 100 ppt. this was done in duplicate. The median lethal concentration was determined using the Probit Analysis. The 24hr LC50 value of the sample and benzene were 4.734 l/ml and 11.01 l/ml, respectively. The same concentrations of the crude extract were tested for antimicrobial activity against Eschirichia coli (Castellani and Chalmers, 1919), Staphylococcus aureus ( Rosenbach, 1884), Bacillus subtilis (Cohn, 1872) and Pseudomonas aeruginosa ( Schroeter, 1872). Little or no activity against the selected bacteria was observed

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Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	University Library	Non-Circulation	LG993.5 2001 B4 B84 (Browse shelf(Opens below))	Available		3UPML00020847
Thesis	University Library	Reference/Room-Use Only	LG993.5 2001 B4 B84 (Browse shelf(Opens below))	Available		3UPML00010969

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Thesis (BS Biology) -- University of the Philippines Mindanao, 2001

A crude extract from the seeds of Moringa oleifera Lam locally known as malunggay was obtained using chloroform as solvent and twice purified using benzene. The final crude extract was obtained from the benzene layer after evaporation at room temperature. Cytotoxicity test using brine shrimp lethality was done with the extract diluted to the following concentrations: 1 ppt, 10 ppt and 100 ppt. this was done in duplicate. The median lethal concentration was determined using the Probit Analysis. The 24hr LC50 value of the sample and benzene were 4.734 l/ml and 11.01 l/ml, respectively. The same concentrations of the crude extract were tested for antimicrobial activity against Eschirichia coli (Castellani and Chalmers, 1919), Staphylococcus aureus ( Rosenbach, 1884), Bacillus subtilis (Cohn, 1872) and Pseudomonas aeruginosa ( Schroeter, 1872). Little or no activity against the selected bacteria was observed

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