Initial studies on in vitro germination and early seedling growth of Nepenthes truncata MACF / (Record no. 335)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01935nam a2200205 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00000009309 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220921142333.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2003 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 M37 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Maquilan, Mary Ann D. |
| 245 00 - TITLE STATEMENT | |
| Title | Initial studies on in vitro germination and early seedling growth of Nepenthes truncata MACF / |
| Statement of responsibility, etc. | Mary Ann D. Maquilan |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2003 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 71 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2003 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A protocol for in vitro seed germination of the endangered Philippine pitcher plant, Nepenthes truncate, was developed from a series of seed sterilization trials and germination experiments involving three factors: seed pretreatments, culture medium and salt concentration. Germination studies were done in independent but simultaneous experiments using flasks and jars. Highest efficiency of seed sterilization (90.8%) was obtained by a combination of 70% isopropyl alcohol, 10% commercial bleach, and 3%hydrogen peroxide. This procedure resulted in 83.7% germination based on a sample size of 520 cultures. Germination in in vitro culture was achieved in 18 to 74 days. A previous study reported germination time of 28 to 180 days. Soaking in sugar solution could increase the initial germination percentage. The treatment that gave the highest final germination percentage was a combination of seed hardening and Murashige and Skoog (92.4%) or Knudson C medium (92.2%). Flasks capped with paper and aluminum sheets produced healthier seedlings than jars with unvented metal caps. Knudson C gave superior effects on seedlings that eventually became chlorotic of necrotic and were not able to supervise longer in cultures. Abnormalities were most severe in seedlings exposed to three-fourth to full concentration of MS salts. |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-09-21 | UAR-T-gd315 | LG993.5 2003 B4 M37 | 3UPML00020731 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
| University Library | University Library | 2022-09-21 | CSM-T-gd850 | LG993.5 2003 B4 M37 | 3UPML00010761 | 2022-09-21 | 2022-09-21 | Thesis |