MARC details
| 000 -LEADER |
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| fixed length control field |
02346nam a2200241 4500 |
| 001 - CONTROL NUMBER |
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| control field |
UPMIN-00000009302 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
UPMIN |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240530162516.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
240530b |||||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
DLC |
| Transcribing agency |
UPMin |
| Modifying agency |
upmin |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
LG993.5 2004 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) |
B4 A43 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Aldaba, Hena S. |
| Relator term |
author |
| 9 (RLIN) |
26032 |
| 245 00 - TITLE STATEMENT |
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| Title |
Effects of different levels of plant growth regulators on callus induction of sago palm (Metroxylon sagu Rottb.) shoot tip / |
| Statement of responsibility, etc. |
Hena S. Aldaba |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Date of publication, distribution, etc. |
2004 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
41 leaves |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (BS Biology) -- University of the Philippines Mindanao, 2004 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Metroxylon sagu Rottb. produces huge amount of starch. The use of tissue culture in sago is beneficial since it has the potential to produce thousands of plantlets in a short period of time. Prior to inoculation to their specific media, they should each pass through sterilization treatment, which minimized the degree of contamination in cultures. Sago shoot tips were first exposed to different commercial chlorine concentrations (5%, 10%, 15%, 20%) at different times of exposure (5, 10, 15, 0 minutes) to find out the optimum combination for sterilization. A high percent recovery was found at 15% concentration at 20 minutes. However, there was no statistical significance between chlorine concentration and time of exposure. Then, the explants were cultured to different media with different concentrations of plant growth regulators, specifically, auxin and cytokinin, for callus initiation. The use of these plant growth regulators enhanced callus formation and shoot elongation. The auxin used was 2, 4-dichlorophenoxyacetic aced (2,4-D) at 0-50, and 100 ppm. The cytokinin used was N6-(2-isopentyl) adenine (2-iP) with concentrations of 0, 0.3, and 0.5 ppm. The optimum treatment combination was treatment 5 and 50 ppm 2,4-D and 0.3 ppm 2-iP. The presence of 2,4-D at this level resulted to a high mean percent callus formation. However, at higher than 50ppm 2,4-D, less callus formation was observed because of its inhibitory effect. Meanwhile, at 0.3 ppm 2-iP showed a high weight increase due to shoot formation. Callus formation and shoot elongation contributed significantly to weight inc |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE |
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| Main curriculum objective |
Undergraduate Thesis |
| Curriculum code |
BIO200, |
| Source of term or code |
BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
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| a |
Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
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| a |
UP |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Thesis |
