Effects of varying pre-insemination handling methods on the motility and acrosome integrity of frozen bulgarian buffalo spermatozoa / (Record no. 267)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02397nam a2200205 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00000009207 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220921140844.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2000 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 L55 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Lim, John Alfred H. |
| 245 00 - TITLE STATEMENT | |
| Title | Effects of varying pre-insemination handling methods on the motility and acrosome integrity of frozen bulgarian buffalo spermatozoa / |
| Statement of responsibility, etc. | John Alfred H. Lim |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2000 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 28 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2000 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study aimed to compare three devised methods of semen storage with regard to semen quality. There were three devised methods assessed. Which involved pre-thawed semen: storing frozen semen in ice (T1), ice + salt (T2), and wrapping semen with damp towel (T3). All the samples were thawed under water bath condition at 370C for 30 seconds and subjected to the respective treatments. Initial observation of motility was done and smearing was followed for observation of acrosome integrity. Observations were done every hour for five hours. The results of the experiment showed that T1 resulted in an increase in sperm motility after five hours of storage. Both T2 and T3 resulted in a decrease in motility after five hours of storage. The motility mean for T1, T2, and T3 were 5,058, 4.771, and 4.02, respectively, with T1 showing the highest motility. Acrosome integrity was not significantly affected by storage under T2 and T3. However, for T1, a decrease in the proportional of normal acrosome was observed up to the third hour. After four hours, a significant increase was observed, although the obtained value was significantly lower (80.57) than that for T2 and T3 with values 85.66 and 85.95, respectively. Except in treatment 1, there was no significant difference in post-thaw motility and acrosome integrity of spermatozoa between bull 1 and bull 2. Ice storage can be a useful storage method to maintain motility, however, further studies must be conducted to determine the effects of handling methods on other physical characteristics of spermatozoa. Storage in ice + salt can also be an alternative handling method because its final motility was significantly higher than the initial, although in the fifth hour. It started to decrease |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-09-21 | UAR-T-gd035 | LG993.5 2000 B4 L55 | 3UPML00020754 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
| University Library | University Library | 2022-09-21 | CSM-T-gd668 | LG993.5 2000 B4 L55 | 3UPML00010991 | 2022-09-21 | 2022-09-21 | Thesis |