Optimization of a Microwell cytotoxicity assay using Artemia salina (brine shrimps) / (Record no. 262)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01430nam a2200193 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00000009202 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220921140843.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2000 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 M35 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Malicsi, Myra Sharon . |
| 245 00 - TITLE STATEMENT | |
| Title | Optimization of a Microwell cytotoxicity assay using Artemia salina (brine shrimps) / |
| Statement of responsibility, etc. | Myra Sharon Malicsi |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2000 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 25 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2000 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The microwell cytotoxicity assay using Artemia salina (brine shrimps) was done as follows: (1) Artemia salina cysts, 0.25 grams, was hatched in 100 ml artificially prepared seawater supplemented with 0.006 grams of yeast extract, (2) continuous moderate aeration was provided in the culture media, (3) serial dilutions of chloramphenicol, cyclohexanone and vincristine were made in the well of the 96-microwell plate, (4) a suspension containing 10-15 nauplii was added tp each well. (5) after 24 hours of hydration, the number of dead nauplii per well was counted, and (6) media lethal concentration was determined using the Probit analysis. It was found that efficiency and rate of hatching increased with salt concentration in the culture media. The 24 hr. LC50 of chloramphenicol, cyclohexanone and vincristine were 39450.12 mg/ml, 5007.907 mg/ml and 1023.712 mg/ml, respectively |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-09-21 | UAR-T-gd040 | LG993.5 2000 B4 M35 | 3UPML00020854 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
| University Library | University Library | 2022-09-21 | CSM-T-gd673 | LG993.5 2000 B4 M35 | 3UPML00010998 | 2022-09-21 | 2022-09-21 | Thesis |