Optimization of protocols for the use of Banana SSR markers in genome analysis of Abaca (Musa textilis Nee) / (Record no. 215)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01894nam a2200205 4500 |
| 001 - CONTROL NUMBER | |
| control field | UPMIN-00000009141 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220921140839.0 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DLC |
| Transcribing agency | DLC |
| Modifying agency | upmin |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | LG993.5 2002 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) | B4 S65 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Solito Melissa T. |
| 245 00 - TITLE STATEMENT | |
| Title | Optimization of protocols for the use of Banana SSR markers in genome analysis of Abaca (Musa textilis Nee) / |
| Statement of responsibility, etc. | Melissa T. Solito |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2002 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 44 leaves |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (BS Biology) -- University of the Philippines Mindanao, 2002 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Abaca (Musa textilis Nee) is an endemic species to the Philippines. The Philippines is considered as the center pf origin of abaca. It holds the largest collection of abaca accessions in the world. However, due to the decline in the economic importance of the crop, very limited funds are available for its research, and almost nil for biotechnology. Being closely related to edible bananas, it is possible that advances in research on banana may be used in abaca. Thus, this study was conducted to develop the optimized conditions for sample sequence repeat (SSR) of microsatellite analysis in abaca using SSR primers previously developed for banana. The conditions that were manipulated for the optimization included annealing temperature, template DNA concentration, number of repeat cycles in the PCR process and the run time of PAGE. The optimized conditions were tested using 28 banana SSR primer pairs. Out of the 28 banana SSR primer pairs tested, only five primers worked well for abaca. The optimized conditions for SSR analysis of abaca are as follows: 52-600C annealing temperature, 30-35 PCR cycles, 20-30 ng template DNA concentration, and 45 min PAGE run. The five primers identified that worked well for abaca are: Ma1/016, Ma1/032, Ma2/4, Ma 3/103, and Ma3/139 |
| 658 ## - INDEX TERM--CURRICULUM OBJECTIVE | |
| Main curriculum objective | Undergraduate Thesis |
| Curriculum code | BIO200, |
| Source of term or code | BSB |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | Fi |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| a | UP |
| Withdrawn status | Lost status | Damaged status | Status | Collection | Home library | Current library | Date acquired | Accession Number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| University Library | University Library | 2022-09-21 | UAR-T-gd379 | LG993.5 2002 B4 S65 | 3UPML00020794 | 2022-09-21 | 2022-09-21 | Thesis | ||||||
| University Library | University Library | 2022-09-21 | CSM-T-1072 | LG993.5 2002 B4 S65 | 3UPML00011012 | 2022-09-21 | 2022-09-21 | Thesis |